The mission of the University of Minnesota Department of Chemistry Mass Spectrometry Laboratory is to bring state-of-the-art mass spectrometry expertise, methodology, and instrumentation to the research and clinical infrastructure of the University of Minnesota. Dr. Joseph Dalluge joined the University of Minnesota in 2009 to direct and expand an existing MS facility. The Mass Spectrometry Laboratory currently occupies about 2,000 square feet of laboratory space on the 1st floor of Kolthoff Hall as part of the Leclaire-Dow Chemical Instrumentation Facility that also includes the X-Ray Crystallography Laboratory and the NMR Laboratory. Support for the facility comes from service fees charged to investigators and direct institutional support from the University of Minnesota. In addition to the mass spectrometry core services provided, the Mass Spectrometry Laboratory has established close research collaborations with a number of departments within the University, including Chemistry, Biological Sciences, and Medicine, and is part of the Center for Bioanalysis of Molecular Signaling.
MALDI-MS is a powerful tool for analyzing a variety of different analytes including small molecules (metabolites), lipids, nucleic acids, peptides, proteins, and polymers ranging in molecular mass from 100 Da up to 100 kDa or more. For analysis by MALDI, a sample is mixed or coated with an energy-absorbing matrix and then irradiated with a laser beam (typically a Nd:Yag laser).
MALDI-MS is a powerful tool for analyzing a variety of different analytes including small molecules (metabolites), lipids, nucleic acids, peptides, proteins, and polymers ranging in molecular mass from 100 Da up to 100 kDa or more. For analysis by MALDI, a sample is mixed or coated with an energy-absorbing matrix and then irradiated with a laser beam (typically a Nd:Yag laser).
The Bruker BioTOF II is a reflectron ESI-TOF instrument capable of 20,000 resolving power (FWHM) in its multipass mode of operation. The instrument has ESI and APCI capabilities. Samples are introduced via syringe pump. The BioTOF II is used primarily for hands-on ESI accurate mass measurements.
Mass Cytometry combines the advantages of single cell high speed analysis common to conventional flow cytometry with the ability to resolve over 100 metal probes with minimal signal overlap common to atomic mass spectroscopy, thereby providing researchers with an unparalleled ability to phenotypically and functionally profile cells from normal and diseased states.
The Waters Acquity UPLC/Synapt G2 QTOF combines ultra performance liquid chromatography with accurate mass MS, MS/MS and MS(e) with the ability to perform simultaneous ion mobility separation. This allows for highly selective and sensitive characterization of compounds in complex mixtures.
The Waters Acquity UPLC/TQD combines ultra performance liquid chromatography with photodiode array (UV) and triple quadrupole mass spectrometric (MS/MS) detection for highly selective and sensitive characterization of compounds in complex mixtures.
MRFN.org is supported by the MRSEC Program of the National Science Foundation. Image in the header is courtesy of CRISP MRSEC.
