The newly upgraded Bruker AVANCE500 spectrometer is a narrow bore, high resolution, nmr spectrometer that is being used for both solution and solid-state NMR spectroscopy. It is a four channel spectrometer that operates at 11.7T (1H frequency of 500MHz). It is equipped with various double resonance Broardband Probeheads for both solution (10mm BBO 1H/X Double Resonance Probe) and solids (2.5mm, 4mm, and 5mm MAS 1H/X Probes), Triple-Resonance/Triple-Gradients Probe for biological solution NMR, Triple/Resonance Transmission Line Probe (under building) for solids, and 4mm hr-MAS 1H/13C z-gradient Probe for macromolecules and oriented media in solution. See the Table for more probe details.

Click here for Probe Features of AVANCE500

The AVANCE500 spectrometer has comprehensive modern NMR capabilities and covers a wide range of NMR experiments in both solution- and solid-state, as shown below.

• Solution NMR:
• Everything which can be done on AVANCE200, and the same 1D NMR and 2D NMR operation procedures can be followed for solution NMR.
• Features:
• 4-Channels: 1H, X, Y, and Z
• Variety of probes (see Probe List)
• Digital Filtering and Oversampling
• Deuterium Decoupling (to be installed) and Observe
• 0.05¡ phase stability, 0.001 Hz frequency resolution, ±0.1 °C temperature control, and linear power amplifiers
• 3-axis Gradients:
• DOSY for diffussion measurement
• Gradient Shimming
• Gradient Solvent Suprresion (WET,MA,WATERGATE)
• etc.
• Macromolecular Study:
  Proteins, DNAs, RNAs, Protein-DNA(RNA) complexes, Lipid Bilayers, etc.
• 3D Structure and Dynamics:
  NOESY, HSQC-NOESY, TOCSY-NOESY, HNCA, HNCACB, HNCO, TROSY, HCCH-TOCSY, and all other experiments for biological NMR

• Solid-state NMR:
  • Super High Sample Spinning Rate up to 35 kHz
  • High Power Amplifiers: 1kW for both 1H and BB channel
  • Fast Digitizer: 12 bit FADC with 10 MHz sampling rate
  • Sample Changer (without removing probe from magnet)
  • Variable Temperature (see Table for Probes)
  • Samples: Polymer (Plastic), Biopolymer (Protein, Lipid), Catalyst (Zeolite), etc.
  • Application:
  • Activation Energy, Chemical Shift, CSA, Quadrupolar Parameters(CQ, h)..
  • Correlation, Exchange, Motion, Relaxation, ...
  • Spectral Editting, Connectivity, Distance, Geometry, Orientation, ...
  • Experiments for I=1/2 Nuclei (see NMR Periodic Table):
    BD, CPMAS, CRAMPS (1H Observe), 19F-Observe/Decoupling, (CPEDIT, SS-APT), (MAS-J-HMQC, INADEQUATE, HETCOR, J-HETCOR), (C7, DRAW, MELODRAMA, R2,REDOR, RFDR, TEDOR), etc.
  • Experiments for I>1/2 Nuclei (see NMR Periodic Table):
    CP-MQMAS, MAS, MQMAS, MQMAS-HETCOR, MQ-REDOR, QUADECHO, REAPDOR, RIACT, TRAPDOR, etc.

  For more details of individual nucleis, please see:

  • NMR Frequecy
  • NMR Periodic Table
  • Properties of Nuclei

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